October 2014 issue | 3(2) 2014
Research Article Open Access
In vitro accumulation of lead nitrate in safflower seedling and its impact on plant protein
Nidhi Srivastava1*, Sameer S. Bhagyawant2
1Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India
2SOS in Biotechnology, Jiwaji University, Gwalior, Madhya Pradesh, India
Abstract
The changes in the morphology, biochemical, antioxidant enzyme activity and protein profile in Safflower were investigated. The plant was treated with different concentrations (100 and 150µM) of Pb(NO3)2 to find the effect of heavy metal stress in Carthamus tinctorius and changes in growth and oxidative stress in one-week and two-week-old plant were examined. The present study has revealed that the presence of heavy metal stress in the plant seedlings causes significant effect in morphological characters, with a decrease in the leaf/root color, size, length and number of leaf/root as compared to control. Similarly, the carbohydrate content was decreased in both leaf and root explants while pigments chlorophyll (a+b) decreased in leaf explants. Proline and polyphenolic compounds were increased compared to control which indicates the excellent antioxidative ingredient to protect damage induced by free radicals. The protein profile of safflower (Carthamus tinctorius) strongly influenced by severe heavy metal stress (100µM and 150µM). It was analyzed through SDS-PAGE and it was found that the protein concentration decreased with increase in metal treatment.
Pages 39-46 | Full Text PDF
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Research Article Open Access
Influence of salinity and adaptive compounds on growth parameters, carbohydrates, amino acids and nucleic acids in two cultivars of Vicia faba contrasting in salt tolerance
Mahmoud Elbaz Younis * and Shaimaa Mohamed Nagy Tourky
Botany Department, Faculty of Science, University of Mansoura, Mansoura, Egypt
Abstract
Salinization of two cultivars of broad beans with 50 and 300 mM NaCl, in general, induced significant reductions in all growth parameters, as compared with control values determined in seedlings after 14 days from being set to germinate. The magnitude of reduction was more pronounced in the salt-sensitive than in the salt-tolerant cultivar, especially with 300 mM NaCl. Thus, the calculated relative growth rate (RGR) in response to treatment with the high level of NaCl, in the salt-sensitive cultivar, was lower than that in the salt-tolerant one. Furthermore, significant increases in the contents of glucose, proline (Pro) and glycine associated with significant decreases in the contents of sucrose, polysaccharides, total saccharides, DNA and RNA, as compared with control levels, were obtained. The use of the optimum concentration of each of the adaptive compounds, either alone or in combination with each of the salinity levels, induced significant varied increases (in glucose, Pro, glycine, DNA and RNA) or decreases (in sucrose, polysaccharide and total saccharides), in relation to the contents of untreated- or salinized-controls; thus the magnitude of counteraction of the salinity adverse effects in broad bean seedlings being most pronounced with SA.
Pages 47-56 | Full Text PDF
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In vitro accumulation of lead nitrate in safflower seedling and its impact on plant protein
Nidhi Srivastava1*, Sameer S. Bhagyawant2
1Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India
2SOS in Biotechnology, Jiwaji University, Gwalior, Madhya Pradesh, India
Abstract
The changes in the morphology, biochemical, antioxidant enzyme activity and protein profile in Safflower were investigated. The plant was treated with different concentrations (100 and 150µM) of Pb(NO3)2 to find the effect of heavy metal stress in Carthamus tinctorius and changes in growth and oxidative stress in one-week and two-week-old plant were examined. The present study has revealed that the presence of heavy metal stress in the plant seedlings causes significant effect in morphological characters, with a decrease in the leaf/root color, size, length and number of leaf/root as compared to control. Similarly, the carbohydrate content was decreased in both leaf and root explants while pigments chlorophyll (a+b) decreased in leaf explants. Proline and polyphenolic compounds were increased compared to control which indicates the excellent antioxidative ingredient to protect damage induced by free radicals. The protein profile of safflower (Carthamus tinctorius) strongly influenced by severe heavy metal stress (100µM and 150µM). It was analyzed through SDS-PAGE and it was found that the protein concentration decreased with increase in metal treatment.
Pages 39-46 | Full Text PDF
………………………………………………………………………………………………………………………………………………………………………………..
Research Article Open Access
Influence of salinity and adaptive compounds on growth parameters, carbohydrates, amino acids and nucleic acids in two cultivars of Vicia faba contrasting in salt tolerance
Mahmoud Elbaz Younis * and Shaimaa Mohamed Nagy Tourky
Botany Department, Faculty of Science, University of Mansoura, Mansoura, Egypt
Abstract
Salinization of two cultivars of broad beans with 50 and 300 mM NaCl, in general, induced significant reductions in all growth parameters, as compared with control values determined in seedlings after 14 days from being set to germinate. The magnitude of reduction was more pronounced in the salt-sensitive than in the salt-tolerant cultivar, especially with 300 mM NaCl. Thus, the calculated relative growth rate (RGR) in response to treatment with the high level of NaCl, in the salt-sensitive cultivar, was lower than that in the salt-tolerant one. Furthermore, significant increases in the contents of glucose, proline (Pro) and glycine associated with significant decreases in the contents of sucrose, polysaccharides, total saccharides, DNA and RNA, as compared with control levels, were obtained. The use of the optimum concentration of each of the adaptive compounds, either alone or in combination with each of the salinity levels, induced significant varied increases (in glucose, Pro, glycine, DNA and RNA) or decreases (in sucrose, polysaccharide and total saccharides), in relation to the contents of untreated- or salinized-controls; thus the magnitude of counteraction of the salinity adverse effects in broad bean seedlings being most pronounced with SA.
Pages 47-56 | Full Text PDF
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